Journal article
Micrococcus luteus: a novel lipolytic bacterial strain isolated from dry contaminated habitat in Jeddah, Saudi Arabia


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Subtitle: Micrococcus luteus 2030 : a novel lipolytic bacterial strain isolated from local contaminated soil in Saudi Arabia
Author list: Al-MAKISHAH N.H., RAI A.K., NEAMATALLAH A.A. and MABROUK A.M.
Publisher: POLSKIE TOWARZYSTWO LESNE
Publication year: 2019
Journal: Sylwan
Volume number: 163
Issue number: 6
Start page: 132
End page: 152
Number of pages: 21
ISSN: 0039-7660
Web of Science ID:
PubMed ID:
Scopus ID:
Languages: English-United States


A novel Lipolytic Micrococcus strain isolated from dry contaminated habitat in Jeddah, Saudi 

Arabia. Lipolytic, or lipase producing, bacteria are a promising for future study, because lipases 

employed in a transesterification reaction cause the production of biodiesel. This study examines 

various aspects of biofuel production employing lipolytic bacteria isolated from soil containing 

deferent waste residue, or organic feedstock. Specifically, nine bacterial strains isolated from 

different locations in Jeddah, Saudi Arabia, including one novel lipolytic bacteria. Characterization of 

the strains, including their growth pattern, lipolytic transesterification activity, and identification by 

gene sequencing is conducted. The findings obtained by employing next-generation sequencing of 

16S rDNA reveal that the maximum lipolytic activity in one bacterial strain, ‘2030’, characterize 

this as a highly efficient strain. The identification results evidence strain 2030 as Micrococcus 

luteus (M. luteus 2030) by accession number- MK300598. A phylogenetic analysis of M. luteus 

strain 2030 shown a highly harmony with similar 47-strain sequences available in the National 

Center for Biotechnology Information (NCBI) GenBank database, employing MEGA 6 software. 


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Last updated on 2019-11-09 at 12:38